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KMID : 0370220080520020125
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Yakhak Hoeji
2008 Volume.52 No. 2 p.125 ~ p.130
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Reactivity of Prototype Foamy Virus Integrase to the Mutants of the Highly Conserved Terminal Sequence of U5 LTR
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Hyun U-Sok
Lee Dong-Hyun
Ko Hyun-Tak
Shin Cha-Gyun
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Abstract
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The long terminal repeat (LTR) of retroviral DNA genome plays an important role in the integration process by providing substrate recognition site for viral integrase (IN). The dinucleotide CA near the 3’-end of the LTR termini is completely conserved among retoviruses. In order to study specificity of interaction between prototype foamy virus (PFV) IN and its U5 LTR DNA, the effect of mutagenesis of the CA sequence was investigated by studying reactivity of PFV IN to the mutant LTR substrates. Replacement of only the C or the A allowed 60 to 100% of the reactivity of the wild type LTR substrate. In addition, replacement of the C and the A showed 50 to 80% of the reactivity of the wild type LTR substrate, indicating that PFV IN has less specificity on the conserved CA sequence when it is compared to the other retroviral INs. Therefore it is suggested that PFV IN is less dependent on the conserved sequence of LTR termini for its enzymatic reaction.
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KEYWORD
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Integrase, Foamy virus, U5 LTR, Conserved
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